Cryotubes & Cell Freezing

barcoded cryotubes

Cryotubes

One of the important but often overlooked aspects of cell freezing is the cryotube that is used. If a low-quality cryotube is used, liquid nitrogen can enter the tube during storage. While this may not damage the samples while in storage, as they are in a cryogenic environment anyway, it is a problem when you go to thaw the cells.

Since frozen cells are generally thawed quite rapidly, the liquid nitrogen in the tube will rapidly vaporize, causing a rapid increase in pressure inside the cryotube that most often results in the explosion of the tube. Such a situation can arise due to the use of poor quality cryotubes or due to user error. In the case of user error, the cap of the cryotube is generally either left too loose or over-tightened, damaging the threading.

Another important aspect of how cryotubes impact good cell freezing technique is sample organization. Markers and pens do not always prove to be a reliable method of marking a cryotube. To ensure that samples are accurately managed, some cryotubes employ bar codes. Bar codes that are built into the cryotube design cannot wear or rub off like ink, and are the required in many biobanking facilities.

For barcoded cryotubes that are consistently high-quality, we invite you to view our TruCool Cryotubes. TruCool CryoTubes are leak-proof, having a screw-cap with a thermally fused gasket. Instead of the usual silicone o-ring, which is usually not affixed to the tube or cap, this gasket is a co-molded thermoplastic elastomer that is actually fused onto the cryotube.

TruCool Cryotubes come in sizes from 1ml to 5ml and in either internal or external threading. All TruCool cryotubes are sterile and DNA-, DNase-, RNase-, Pyrogen- and ATP-free.

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